Preparation of monomethine cyanine dyes as noncovalent labels for nucleic acids
نویسندگان
چکیده
منابع مشابه
Rigid cyanine dye nucleic acid labels.
Cyanine dyes attached to DNA via a rigid linker show useful fluorescence and FRET properties without altering the stability of duplex DNA.
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Asymmetric monomethine cyanines have been extensively used as probes for nucleic acids among other biological systems. Herein we report the synthesis of seven monomethine cyanine dyes that have been successfully prepared with various heterocyclic moieties such as quinoline, benzoxazole, benzothiazole, dimethyl indole, and benz[e]indole adjoining benz[c,d]indol-1-ium, which was found to directly...
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Electron paramagnetic resonance (EPR) spectroscopy is widely used to study free radicals or paramagnetic centers associated with biopolymers. With the advent of pulsed EPR methods, which allow accurate distance measurements between 20 and 80 , structures of biopolymers have increasingly been interrogated by this technique. Some of the advantages of EPR spectroscopy over other structural techniq...
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Cyanine dyes have been shown to undergo reversible photoswitching, where the fluorophore can be switched between a fluorescent state and a dark state upon illumination at different wavelengths. The photochemical mechanism by which switching occurs has yet to be elucidated. In this study, we have determined the mechanism of photoswitching by characterizing the kinetics of dark state formation an...
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Selected developments and trends in stains, labels and strategies for detecting and measuring nucleic acids (DNA, RNA) and related molecules [e.g. oligo(deoxy)nucleotides, nucleic acid fragments and polymerase chain reaction products] are surveyed based on the literature in the final decade of the 20th century (1991-2000). During this period, important families of cyanine dyes were developed fo...
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ژورنال
عنوان ژورنال: Dyes and Pigments
سال: 1999
ISSN: 0143-7208
DOI: 10.1016/s0143-7208(98)00046-1